Why use CCC
CCC complimentary HPLC




Natural Product Bioactitives
Isocratic vs Step Gradient
Novel Alkaloids


Polyphenols
Engine Oil Additives, Isocratic
Effect Coil Volume
Why need Gradients in CCC
Comparison to Flash Chrom
Which Biphasic Solvents
Linear Gradients
pH Modification Part Coeff
Step Gradient
Linear Gradient RP
Comparison NP to RP
Unknown Bioactivites
Engine Oil Additives :Gradient
LC-FID

PILOT / PROCESS SCALE : - GRAMS TO MUTIPLE TONNES

THE QUATTRO PILOTPREP :

The Quattro LabPrep and PilotPrep have a bench footprint of 710 mm as standard, but custom units with larger footprints, and larger capacity coils are available, email sales / technical enquires for more details

The LabPrep and PilotPrep are effectively from a CCC standpoint, the same instrument, with both having the same options of speed, coil configuration, temperature control etc options.

The difference being that for intrinsically safe operation the PilotPrep has no aluminum in it's construction and it has a control / motor etc electrical design to meet intrinsically safe operational standards.

The unit can only be purchased with a pair of matched bobbins, which can have 1, 2, 3 or 4 coils on each bobbin.

Therefore a single Quattro instrument can be performing up to eight similar analyses on different samples or eight different analyses on one sample or any combination. This facility is designed to provide rapid sample throughput in natural product and combinatorial screening laboratories

Coils can be of user specified id and material ( PTFE or STAINLESS, others on request ), with multiple matched sets available at time of purchase, or added as required.

Depending on coil material and id chosen; capacity can range from 600 ml to several litres.

Typical loadings on this instrument would therefore be sub milligram to 10 to 20 grams plus ( although certain Quattro users have achieved 20 gram separation on a 600 ml Quattro LabPrep Mk2 and would therefore be able to achieve 100 g loading on a 3000 ml Quattro PilotPrep Mk 4.

To find out more on the potential of a Quattro PilotPrep, email sales / technical enquirers direct from the home page

All Quattro LabPrep and PilotPrep CCC's are temperature controlled for reproducible CCC chromatography

There are over 60 patents for different concepts of CCC, all retain solvents in a different manner, percentage, etc and require different methods to be developed for the same compound preparation.

Even if only consider the Ito ' J ' Type, that is, the Quattro CCC Type, you can have different manners to achieve the required ' G ' Force differential

A small sun and planetary radius but very high speed or progressively larger sun and planetary radii, with progressively reducing speed of rotation will both work as Counter Currents.

Unfortunately, there are a near infinite number of biphasic solvent system combinations, and whilst some will transfer methodology from one size ' J ' Type to the next, a very large number of solvent systems cannot be scaled from one size ' J ' Type to the next.

EXPERIMENTATION HAS SHOWN THAT WITH A SINGLE QUATTRO LABPREP, KEEPING ALL PARAMETERS THE SAME EXCEPT OPTIMISING FLOW AND LENGTH OF TUBING ( VOLUME OF COIL ) THAT IF THE BORE IS MAINTAINED, AND FLOW IS INCREASED TO THE LIMIT OF THAT SEPARATION, THEN FOR COLUMN LENGTH INCREASE, SCALE UP IS TOTALLY PREDICTABLE.

IF, HOWEVER, ON THAT SAME INSTRUMENT, AN ATTEMPT IS MADE TO INCREASE FLOW BY USE OF LARGER INTERNAL DIAMETER TUBING, NO GUARANTEE OF SUCCESSFUL SCALE UP CAN BE PREDICTED, FURTHER EXPERIMENTATION IS REQUIRED TO FIND IF THIS ID OF TUBING WILL WORK AT ANY FLOW RATE FOR MANY CRITICAL SOLVENT SYSTEMS.

Publications inferring that changes in sun / planetary radii, speed, internal bore diameter etc can be predictably, mathematically extrapolated mislead by accidental or deliberate choice of NON-CRITICAL solvents and solutes.

There is therefore a need to define all critical parameters in CCC

All Quattro CCC are designed to have acceptable constancy in planet / sun radii and can be purchased with a range of coil internal diameters or coil material type ( PTFE, Stainless Steel etc ). Any Quattro CCC that gives an acceptable resolution at the user defined optimum bore / flow rate / speed combination for a given id / coil material can then be scaled up to any production requirement by use of the appropriate number of Quattro ProcessPrep modules.

THE QUATTRO PROCESSPREP :

The Quattro ProcessPrep is of a modular design to allow flexibility of process design.

IT IS EFFECTIVELY THE STANDARD ROTOR / BOBBIN DESIGN BUILT IN STAINLESS STEEL WITH NO ALUMINIUM IN ITS CONSTRUCTION, SO IT CAN BE USED IN INTRINSICALLY SAFE PROCESS ENVIRONMENTS

STANDARD PROCESSPREP CCC HAVE NO CASEWORK BUT THIS IS AN OPTION IF REQUIRED, SEE PHOTOGRAPH.

The module can be fitted by the user into an intrinsically safe room, which then becomes, its safety cell, not permitting access to staff while instrument rotating, plus the room will control temperature and solvent fume venting, and all user specified required safety features

When operating many modules in series or parallel in a single room, Quattro ProcessPrep modules becomes an extremely cost effective method of compound purification, especially as all modules could, if required, run from a single motor via an appropriate drive shaft system.

The motor could be outside of the CCC module containment area and need therefore not necessarily be of intrinsically safe design if appropriately positioned.

Quattro ProcessPrep is ideal for simulated moving bed manufacture / purification procedures, and given that a single rotor costing less than $12,000 could prepare 20 to 100 grams and often uses a half to a fifth of the solvent of process hplc, we believe that manufacture by CCC has a bright future. Contact our sales / technical service directly from the home page for more information.


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