The considerable effectiveness of an appropriate linear gradient can be see by comparing the complexity of the gradient hplc bioactive natural product extract, Slide 23 to the extremely high purity of the Quattro CCC purified bioactive ingredient shown it Slide 24. This slide also shows that 95% of this active ingredient was contained in a single 4 ml fraction which from the UV trace of the CCC run Slide 22 appeared to be identical to the previous fraction, which in fact contained only a minor trace of this bioactive component.