Whilst linear gradients
have been successfully used in many applications including one step isolation
of novel bio actives from untreated plant extracts as shown in Slide
22 a difficulty arises when trying to extrapolate from the linear
gradient elution position to a suitable isocratic elution system.
The considerable effectiveness of an appropriate linear gradient can be
see by comparing the complexity of the gradient hplc bioactive natural
product extract, Slide 23 to
the extremely high purity of the Quattro CCC purified bioactive ingredient
shown it Slide 24. This slide
also shows that 95% of this active ingredient was contained in a single
4 ml fraction which from the UV trace of the CCC run Slide
22 appeared to be identical to the previous fraction, which in
fact contained only a minor trace of this bioactive component.