Slide 14 shows partially immiscible solvents where no relative density differences between upper and lower layer occurs as gradient solvent modifier is added and an example of a chloroform gradient where an inversion of an upper aqueous layer to a lower aqueous layer occurs as the organic gradient modifier is progressively added.
Examples on Slide 13 have no application in CCC whilst the first example on Slide 14 is an usable, predictable gradient throughout the gradient. The second chloroform gradient of Slide 14 which has an inversion of its upper aqueous layer to a lower aqueous layer as the methanol gradient modifier is added, can only be used either before or after the inversion point, as the inversion would void the stationary phase in HSCC