Introduction to use of isocratic biphasic solvent systems in HSCCC
In HSCCC a biphasic
liquid / liquid solvent system is used ( See CCC Introduction on Home
Page ). The mobile phase may be either the more polar solvent ( reverse
phase ) or less polar solvent ( normal phase ) with their respective partner
phases as the stationary phase. Indeed it is possible to change from one
mode to the other at any stage during a run.
It is often best to do a HSCCC literature review ( See Literature References
on Home Page ) to try to find solvent systems, which have been used for
similar components to your sample prior to commencing optimization of
any HSCCC method.
If none exists a strategy similar to that discussed in the following gradient elution sections should be utilized.
Slide 1 gives the structure of a variety of bioactive natural product polyphenols, which are then shown in Slide 2 resolved in a preparative isocratic reverse phase application using a Quattro CCC